DMEM (high glucose)
Thermo Fisher Scientific
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Cell Line Engineering Stack
Cell line engineering
Generate stable cell lines for protein expression and screening.
Complete workflowDMEM (high glucose)
Thermo Fisher Scientific
Neon Transfection System
Thermo Fisher Scientific
Cell Culture Flask (T75)
Corning
Countess 3 Cell Counter
Thermo Fisher Scientific
Cell Culture Incubator
Eppendorf
Centrifuge 5424 R
Eppendorf
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Antibody characterization by flow cytometry
5 components · Updated 3 months ago
Flow cytometryAntibody characterization
View StackSee how the stack changed over time.
See how the setup changed as new products, decisions, and outcomes emerged.
- v1Jun 2024Initial setupFirst working configuration.
- v2Aug 2024Replaced reagentSwitched to a higher sensitivity antibody.
- v3Jan 2025Added softwareIncluded analysis software for improved gating.
- v4Apr 2025Updated protocolRefined staining protocol based on community feedback.
- v5Jul 2025Current setupLatest, optimized configuration.
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Instruments & EquipmentReceipt Stack · 6 components
Neon Transfection System
Model / version: MPK5000
Thermo Fisher ScientificUsed for 9 monthsNon-GxP / Research / Academic
Use Case
Transient transfection of suspension-adapted HEK293 cells for small-scale protein expression and plasmid screening.
Experience
Reliable for routine transfections once the electroporation settings were optimized for the cell line. Setup was straightforward, throughput was good for development work, and performance was consistent across repeated runs.
Warn a Peer
Expect some optimization up front. Cell density, buffer condition, and pulse settings can noticeably affect viability and expression, so don’t assume the default program will be optimal for every cell line.
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Instruments & Equipment
“Easy to maintain in our lab.”
Reagents & Consumables
“Consistent lot-to-lot performance.”
Software & Data Tools
“Intuitive and saves time.”
Services & CROs
“Great collaboration and communication.”
Vendors & Suppliers
“Fast delivery and excellent support.”
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